Mutual regulation between microRNA-373 and methyl-CpG-binding domain protein 2 in hilar cholangiocarcinoma

Figure 3 Methylation of CpG island regulates the expression of miR-373. A: Promoter luciferase reporter gene assay for miR-373. Prior CpG methylated resulted in dramatic decrease of luciferase activity of pGL4-m373-prom compared with pGL4-u373-prom (^bP < 0.01 vs control); B: miR-373 expression was reactivated by epigenetic reagents of 5’-Aza-2-CdR or combination with trichostatin A (TSA). ^dP < 0.01 vs untreated QBC₉₃₉.