Analysis of hepcidin expression: In situ hybridization and quantitative polymerase chain reaction from paraffin sections

Figure 3 In situ hybridization for hepcidin. A surgical specimen was stained with hematoxylin-eosin (A: × 100, B: × 200, C: × 400), and also subjected to immunohistochemistry (D: × 100, E: × 200, F: × 400) and in situ hybridization (G: × 100, H: × 200, I: × 400). Hematoxylin-eosin staining shows cancer tissues surrounded by non-cancerous hepatic lobules. Immunohistochemistry shows positive staining for hepcidin in the non-cancerous hepatic lobules (indicated as yellow in the figures). In situ hybridization for hepcidin shows clear expression of hepcidin mRNA in non-cancerous tissues (blue in the figures). Squares represent magnified areas.