Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in decreased secretion of IL-6

Figure 3 Mitogen activated protein kinases pathway involved in interleukin 17A induced interleukin 6 expression. A: Secretion of interleukin (IL)-6 in hepatic stellate cells (HSCs) induced by interleukin 17A (IL-17A) was determined using enzyme-linked immunosorbent assay. ^bP < 0.01 vs control; B: IL-6 mRNA expression in HSCs induced by IL-17A (100 ng/mL) was measured by polymerase chain reaction. ^bP < 0.01 vs control; C: Phosphorylation of p38 MAPK and ERK1/2 induced by IL-17A (100 ng/mL) in HSCs was detected using Western blotting; D: Phosphorylation of p38 MAPK and ERK1/2 induced by IL-17A (100 ng/mL) for 15 min was blocked by preincubation with MAPKs inhibitors, SB-203580 (1 μmol/L in DMSO, 30 min) and PD-98059 (10 μmol/L in DMSO, 1 h); E: IL-6 mRNA expression induced by IL-17A (100 ng/mL) for 3 h exposure was inhibited by preincubation with SB-203580 (SB) and PD-98059 (PD) for the same time period as above. ^aP < 0.05, ^bP < 0.01 vs control. T: Total; P: Phosphorylation; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; MAPK: Mitogen activated protein kinases; ERK: Extracellular regulated protein kinases.