Study of human B7 homolog 1 expression in patients with hepatitis B virus infection

Figure 8 The analysis of the effects of hepatitis B surface antigen on T cell proliferation by carboxyfluorescein diacetate succinimidyl ester labeled mixed leucocyte reaction. A: Myeloid dendritic cells (mDCs) with hepatitis B surface antigen (HBsAg) pretreatment are weak stimulators in the mixed leucocyte reaction (MLR). Approximately 25.4% of T cells are carboxyfluorescein diacetate succinimidyl ester (CFSE)^dim in 1:5 R/S ratio and 12.0% in 1:10 R/S ratio respectively; B: mDCs without HBsAg pretreatment are potent stimulators in the MLR. Approximately 85.1% of the T cells are CFSE^dim in 1:5 R/S ratio and 30.3% in 1:10 R/S ratio respectively. FITC: Fluorescein isothiocyanate. R/S: Responder cell-stimulator cell.