Study of human B7 homolog 1 expression in patients with hepatitis B virus infection

Figure 4 Localization of CD11c and programmed death 1 in liver tissue from acute hepatitis B and chronic hepatitis B virus tolerance patients. A-C: Colocalization of CD11c with SLC, MIP3α and CCR7 by immunofluorescence double staining in liver biopsy specimens of acute hepatitis B (AHB) patients. CD11c (green) is co-localized with SLC (red) (A), MIP3α (red) (B) and CCR7 (red) (C). The 2-color merged panels were shown with colocalization visible in yellow. Original magnification × 200; D: Colocalization of CD8 with programmed death 1 (PD1) by immunofluorescence double staining in liver biopsy specimens of AHB patients. CD8 (green) is co-localized with PD1 (red). Original magnification × 200; E: Inflammatory cells, such as CD8 and CD11c positive cells, can be observed mainly in fibrous septa in the liver tissue of chronic hepatitis B virus tolerance patients. FITC: Fluorescein isothiocyanate; PE: Phycoerythrin; SLC: Secondary lymphoid tissue chemokine; MIP3α: Macrophage inflammatory protein 3 α; CCR: Chemokine (C-C) receptor.