Hepato-biliary profile of potential candidate liver progenitor cells from healthy rat liver

doi:10.3748/wjg.v18.i27.3511

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Jul 21, 2012 (publication date) through Nov 5, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jul 21, 2012; 18(27): 3511-3519
Published online Jul 21, 2012. doi: 10.3748/wjg.v18.i27.3511

Table 1 Primers used for reverse transcription polymerase chain reaction

Gene	Amplicon size (bp)	Primers
Vim	241	F: AAGCAGGAGTCAAACGAATA
		R: GAGCCATCTTTACATTGAGC
Fn	392	F: ACCGTGGAGTATGTGGTTAG
		R: GGTGACACCTGAGTGAACTT
ASMA	385	F: ATGCTTCTGGACGTACAACT
		R: GACTCCATTCCAATGAAAGA
CK8	378	F: TGGAGAATGAGTTTGTCCTC
		R: TGATGTTACGGTTCATCTCA
CK19	287	F: GCCAGTACTTCAAGACCATC
		R: ACTAATTTCCTCCTCGTGGT
HNF4	332	F: CGGATGTGTGTGAGTCTATG
		R: AAAGAAGATGATGGCTTTGA
TAT	275	F: CTGGACAAAACATCCTCATT
		R: GATCTCGTCAGCTAAGATGG
TDO	366	F: CTCCTGGTACAGCAGTTCTC
		R: CTTTTTCGCCTGAATCTTTA
aFP	289	F: AACGTAGCTACCATTGTCGT
		R: CAGTTTCTGGAAAGTGGAAG
Alb	365	F: TTTACGAGAAGCTTGGAGAG
		R: TGTGCAGATATCAGAGTGGA
UGT1A1	369	F: CCATGTTGCTTTTATTAGGG
		R: ACAAAACATGAGCACAGTGA
G6Pase	386	F: GAAGATGTTTCCCTGATGAA
		R: AGTCACCATTACCATTCAGG
GAPDH	315	F: CCACTCAGAAGACTGTGGAT
		R: TGTTGAAGTCACAGGAGACA
CD29	321	F: TTCAATGAACTTGTTGGTCA
		R: AGTGACTGCAAAAATCGTCT
CD44	383	F: AGGATTTCCCCAGAACTTAG
		R: ACAGGTCAAGATGGAAGATG
CD45	321	F: TGAACATACGGATTGTGAAA
		R: TTTGTTCGGACTGTAAGGTT
CD73	341	F: ATAGTCACCTCTGACGATGG
		R: ATTTCATCTGGGTGTCTGAG
CD90	380	F: AAGGAGAAACAGGAAACCTC
		R: ACAGACACAGTCCAACTTCC
CD105	386	F: TACCTCCAAGACACAGATCC
		R: TCTGCATATTGTGGTTGGTA

Fn: Fibronectin; Vim: Vimentin; ASMA: α-smooth muscle actin; CK: Cytokeratin; HNF4: Hepatocyte nuclear factor 4; TAT: Tyrosine aminotransferase; TDO: Tryptophan 2,3-dioxygenase; αFP: α-fetoprotein; Alb: Albumin; UGT1A1: UDP-glucuronosyl transferase 1A1; G6Pase: Glucose-6-phosphatase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Citation: Maerckx C, Scheers I, Tondreau T, Campard D, Nyabi O, Najimi M, Sokal E. Hepato-biliary profile of potential candidate liver progenitor cells from healthy rat liver. World J Gastroenterol 2012; 18(27): 3511-3519
URL: https://www.wjgnet.com/1007-9327/full/v18/i27/3511.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i27.3511