Proteomic analysis of glutathione S-transferase isoforms in mouse liver mitochondria

Figure 4 The calibration curves of mitochondrial glutathione S-transferase proteins. Upper panel in A, B, C, D, E: Examination of the specificity of the five glutathione S-transferase (GST) antibodies, 2 ng of the corresponding GST was loaded in all of the left lanes, and 20 ng of the other unrelated GSTs was loaded in the other four lanes; Lower panel in A, B, C, D, E: The X-axis represents the amounts of recombinant GSTs loaded (ng), and the y-axis represents the relative intensity of the Western blotting, taking the signals generated from the lowest GST amount as the reference. The calibration curves were generated by regression. Insert: The linear equations and R² were generated by regression.