Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner

Figure 5 CD44 engagement is necessary for osteopontin-a induced cell growth in Huh-7 and HeLa cells. (A) Huh-7 or (B) HeLa cells were seeded at a density of 7 × 10⁴ cells per well in a 12-well plate and conditioned media from osteopontin (OPN)-A Huh-7 stable cell lines or Huh-7 cells alone placed onto the cells in conjunction with either anti-CD44 or a control immunoglobulin. Cell numbers were monitored daily using trypan blue exclusion. CM: Conditioned media. ^aP < 0.05.