Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner

Figure 4 CD44 expression is necessary for increased cellular growth due to osteopontin expression. Huh-7, HepG2, Hep3B and HeLa cells were immunostained for 4’,6-diamidino-2-phenylindole (DAPI) and CD44 expression (A); Conditioned media (CM) was removed from osteopontin (OPN)-A, -B, -C and control-expressing Huh-7 stable cell lines in conjunction with Huh-7 cells and placed onto Hep G2 cells (B); HeLa cells (C) or Hep 3B cells (D); cell numbers were monitored daily using a trypan blue exclusion. ^aP < 0.05.