Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner

Figure 3 Osteopontin splice variants increase Huh-7 cell growth. A: Osteopontin (OPN)-A, -B, -C and control-expressing Huh-7 stable cell lines in conjunction with Huh-7 cells, were seeded at a density of 7 × 10⁴ cells per well in a 12-well plate and cell numbers monitored daily using trypan blue exclusion; B: Conditioned media (CM) was removed from OPN-A, -B, -C and control-expressing Huh-7 stable cell lines in conjunction with Huh-7 cells and placed onto Huh-7 cells. Cells were monitored as mentioned above; C: Huh-7 cells were seeded at a density of 7 × 10⁴ cells per well in a 12-well plate and conditioned media from OPN-A, -B, -C and control expressing-Huh-7 stable cell lines placed onto the cells in conjunction with either anti-OPN (OPN Ab) or an control immunoglobulin. ^aP < 0.05.