Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner

Figure 2 Stable expression of osteopontin splice variants demonstrates unique localisation patterns. Huh-7 cell stably overexpressing osteopontin (OPN)-A, -B, -C, or the control, were stained for OPN expression and visualised fluorescently to demonstrate localisation of the three splice variants (A); Stable expression was confirmed using polymerase chain reaction (B), and Western blotting (C). DAPI: 4’,6-diamidino-2-phenylindole.