Phosphoinositide-3-kinase, catalytic, alpha polypeptide RNA interference inhibits growth of colon cancer cell SW948

Table 2 Levels of mRNA expression and protein expression of target genes in different groups normalized by β-actin

Goal gene	CA1	CA2	Negative	Blank
mRNA expression
PIK3CA	0.51 ± 0.04a	0.49 ± 0.03a	0.92 ± 0.01	0.93 ± 0.03
Akt	0.50 ± 0.03b	0.48 ± 0.01b	0.93 ± 0.04	0.92 ± 0.02
c-myc	0.49 ± 0.01a	0.50 ± 0.04a	0.90 ± 0.02	0.91 ± 0.03
cyclinD1	0.45 ± 0.02a	0.51 ± 0.01a	0.96 ± 0.03	0.98 ± 0.01
Protein expression
PIK3CA	0.53 ± 0.01a	0.54 ± 0.02a	0.92 ± 0.03	0.91 ± 0.02
Akt	0.49 ± 0.02b	0.55 ± 0.03b	0.94 ± 0.03	0.95 ± 0.04
c-myc	0.51 ± 0.03b	0.52 ± 0.04b	0.92 ± 0.02	0.95 ± 0.01
cyclinD1	0.54 ± 0.04b	0.56 ± 0.03b	0.93 ± 0.01	0.93 ± 0.03

PIK3CA: Phosphoinositide-3-kinase, catalytic, alpha polypeptide.

^aP = 0.001,

^bP = 0.000 vs negative or blank group.