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©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Jul 14, 2012; 18(26): 3458-3464
Published online Jul 14, 2012. doi: 10.3748/wjg.v18.i26.3458
Published online Jul 14, 2012. doi: 10.3748/wjg.v18.i26.3458
Table 1 Oligonucleotides sequences of primer pairs
| Goal gene | Upstream primer | Downstream primer | PCR frag (bp) |
| β-actin | 5’-TCCTGTGGATCCACGAAACT-3’ | 5’-GAAGCATTTGCGGTGGACGAT-3’ | 330-bp |
| PIK3CA | 5’-CCCTGCTCATCAACTAGGAAACC-3' | 5’-TTGCCGTAAATCATCCCCATT-3' | 160-bp |
| Akt | 5’-GGACAACGCCATCCAGACT-3’ | 5’-GCCAGGGACACCTCCATCTC-3’ | 121-bp |
| c-myc | 5’-TACCCTCTCAACGACAGCAG-3’ | 5’-TCTTGACA TTCTCCTCGGTG-3’ | 477-bp |
| cyclinD1 | 5’-GCCAACCTCCTCAACGACCGG-3' | 5’-GTCCATGTTCTGCTGGGCCTG-3’ | 744-bp |
- Citation: Huang WS, Wang TB, He Y, Chen YJ, Zhong SL, Tan M. Phosphoinositide-3-kinase, catalytic, alpha polypeptide RNA interference inhibits growth of colon cancer cell SW948. World J Gastroenterol 2012; 18(26): 3458-3464
- URL: https://www.wjgnet.com/1007-9327/full/v18/i26/3458.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i26.3458