Omega-3 polyunsaturated fatty acids promote liver regeneration after 90% hepatectomy in rats

Figure 8 Phosphorylation of activation of protein kinase, signal transducer and activator of transcription 3, glycogen synthase kinase-3β. Phosphorylation of activation of protein kinase B (Akt), signal transducer and activator of transcription 3 (STAT3), glycogen synthase kinase (GSK)-3β in the control and the omega-3 polyunsaturated fatty acid (ω-3 PUFA) groups at 12 h, 18 h and 24 h after partial hepatectomy. Liver lysates at 12 h, 18 h and 24 h time points in the quantity of 25 μg per lane were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were transferred to polyvinylidene fluoride membrane and then incubated with specific antibodies. There was stronger phosphorylation of Akt at the 18 h time points and earlier phosphorylation of GSK3β at the 12 h time points compared to the control group. Phosphorylation of STAT3 was stronger and lasted longer at all time points in the ω-3 PUFA group.