Effect of Yiguanjian decoction on cell differentiation and proliferation in CCl4-treated mice

Figure 4 Immunofluorescent colocalization of enhanced green fluorescent protein and α-smooth muscle actin in CCl₄-induced chronic liver injury in bone marrow chimera mice, × 200. EGFP is shown in green and α-smooth muscle actin (α-SMA) in red. A yellow color confirms colocalization in the merged images. The number of EGFP/α-SMA double positive cells was found to increase in all mice after receiving CCl₄ injection and they were mainly found in the areas of scarring. In contrast, the number of EGFP⁺/α-SMA⁺ cells decreased in the YGJ treatment group. EGFP: Enhanced green fluorescent protein; YGJ: Yiguanjian.