Targeting X-linked inhibitor of apoptosis protein inhibits pancreatic cancer cell growth through p-Akt depletion

Figure 1 X-linked inhibitor of apoptosis protein expression analysis and selection of the RNAi target for X-linked inhibitor of apoptosis protein. A, B: The X-linked inhibitor of apoptosis protein (XIAP) protein level and IC₅₀ for Panc-1, SW1990, Mia-paca2 and Bxpc-3 cell lines. R² = 0.87 (Spearman correlation) between IC₅₀ and relative XIAP expression, P < 0.05; C: Relative expression of XIAP mRNA after transfection and selection with puromycin; D: Downregulatory effect of lentivirus-mediated RNAi on XIAP in SW1990 cells. Lv-X1, Lv-X2, Lv-X3 efficiently suppressed XIAP expression. The results also showed that survivin was not affected by any lentivirus. Lv-Xnc, which was transfected with the non-sense lentivirus vector, was set as the calibrator with the relative expression value of “1”. β-actin was used as the internal loading control in three independent experiments. 5-FU: 5-fluorouracil.