Hepatocellular carcinoma and macrophage interaction induced tumor immunosuppression via Treg requires TLR4 signaling

Table 2 Primer sequences and conditions used for reverse transcription-polymerase chain reaction

Gene	Sequence	Product size (bp)	Annealing temperature (°C)
mTLR4	5′-GCTTTCACCTCTGCCTTCAC-3′	259	57
	3′-AGGCGATACAATTCCACCTG-5′
HuTLR4	5′-GAAATGGAGGCACCCCTTC-3′	506	52
	3′-GAATATTCCTTTGCATAGGT-5′
CCL22	5′-AAGACAGTATCTGCTGCCAGG-3′	141	57
	3′-GATCGGCACAGATATCTCGG-5′
IL-10	5′-GGTTGCCAAGCCTTATCGGA-3′	190	60
	3′-ACCTGCTCCACTGCCTTGCT-5′
TNF-α	5′-CATCTTCTCAAAATTCGAGTGACAA-3′	175	58
	3′-TGGGAGTAGACAAGGTACAACCC-5′
β-actin	5′-TCACCCACACTGTGCCCATCTACGA-3′	300	50
	3′-GATAACCGTTGCTCGCCAAGGCTAC-5′

TNF: Tumor necrosis factor; IL: Interleukin; TLR: Toll-like receptor.