Proteome profiling of spinal cord and dorsal root ganglia in rats with trinitrobenzene sulfonic acid-induced colitis

Table 3 Differentially expressed proteins identified in cellular and animal models of inflammatory bowel disease and in clinical samples of inflammatory bowel disease patients

Ref.	Animalmodel	Cell	Patient	Sample	Analytical technique (S)	No. of regulatedproteins	Major findings	Protein name	Change
[68]		Human adenocarcinoma cells Dld-1 exposed to interferon-γ, interleukin-1 and interleukin-6			2D PAGE-MALDI-TOF-MS/MS	5	Protein biosynthesis	Tryptophanyl-tRNA synthetase	Up
							Redox regulation	Indoleamine-2, 3-Dioxygenase	Up
[14]		HT29 Cl.16E cell treated with interferon-γ			1D PAGE-LC ESI/QTOF-MS/MS	7	Structure protein	Histone H2A type-1B	Up
							Metabolic enzymes	Adenosylhomocysteinase	Up
							Redox regulation	Peroxiedonxin-1	Down
			CD patients	Intestinal epithelial cells		14	Structure protein	Histone H1.2, H2A.V, H2B Type 1-C/E/F/G/I, H3 Like, H4	Up
							Chaperone	Heat shock 70 kDa protein 5	Up
							Annexin	Annexin A1	Down
[69]			CD and UC patients	Endoscopic biopsies of colonic mucosa	Multi-epitope-ligand-cartographic immunofluorescence microscopy		Apoptosis	Caspase-3	Down
							Transcription regulation	NF-κ B	Up
[12]			UC patients	Colon biopsies	2D-MALDI-TOF-MS/MS	19	Negative regulation of cell proliferation and DNA replication	Prohibitin (PHB)	Down
							1. TCA Cycle 2. Gluconeogenesis 3. Antioxidant	Mitochondrial malate dehydrogenase (Mdh2)	Down
							Eliminating Peroxides	Thioredoxin peroxidase (Prdx1)	Down
							1. Ion Channel 2. Apoptosis	Voltage-dependent anion-selective channel protein 1 (Vdac1)	Down
							1. Intracellular signal transduction 2. Regulation of transcription, DND-dependent	Nuclear factor of activated T-cells cytoplasmic (NFAT C1)	Up
							Protein folding	Tumor rejection antigen 1 (TRA1)	Up
							Cell adhesion Host-virus interaction	Poliovirus receptor related protein 1 (PVRL1)	Up
[70]			CD and UC patients	Serum	SELDI-TOF-MS	4	Cytokine-mediated signaling	Platelet factor 4	Up
							Chronic inflammation	Myeloid related protein 8	Up
							Fibrin producing and inflammation	FIBA	Up
							Transferase	Haptoglobin alph2	Up
[71]			CD and UC patients	Intestinal epithelial cells	2D PAGE-MALDI-TOF-MS	17	Regulation of GTPase activity	Rho gdp dissociation inhibitor (GDI) α chain	Up
							Differentiation	Nicotinamide phosphor-ribosyltransferase	Up
							Calcium ion binding	Myosin regulatory light chain 2, nonsarcomeric	Up
[72]			CD patients	Serum	RP NANO-LC ESI/Q-TOF MS	8	Immunity	Complement C3	Up
							Blood coagulation	Fibrinogen α chain	Up
							Lipid transport	Apolipoprotein E	Down
[73]			UC patients	Mucosa/ submucosa	2D PAGE- LC-MS/MS	7	Cell adhesion	Protocadherin 17 precursor	Up
							Acute-phase response	α-1-Antitrypsin (precursor)	Up
							Muscle protein	Caldesmon	Up
							Structural molecule activity	Mutant desmin	Up
[74]	DSS treated Balb/C mice			Mucosa	2D-DIGE- MALTI-TOF	5	Isomerase	Protein disulfide isomerase family A, member 3	Down
							Redox regulation	Peroxiredoxin 6 (Prx6)	Down
[13]	TNBS treated SD rats			Lymphocytes	2D-MALDI- TOF-MS/MS	42	Apoptosis-related proteins	PYD and Card domain containing protein	Down
							DNA damage response	Proteasome activator complex subunit 2	Down
							Glycolysis	Phosphoglycerate mutase type B subunit	Down
							Xenobiotic metabolism and cellular defense	Glutathione S-transferase, Pi 2	Down
							Cytokine	Interleukin-12 p40 precursor	Up
							Proteins involved in cell growth, differentiation and signal trransduction	Nucleoside diphosphate kinases β isoform	Up
							Inflammatory factors	Myeloid-related protein	Up
							ATP transduction	ATP-citrate synthase	Up
							Redox regulation	Dismutase	Up
[49]	TNBS treated SD Rats			DRG	2D-MALDI- TOF-MS/MS	26	Xenobiotic metabolism and cellular defense	Glutathione S-transferase P (Gstp1)	Down
							Eliminating peroxides	Peroxiredoxin-1 (Prdx1)	Down
							1. Accelerate the folding of protein 2. Immunosuppression	Peptidyl-prolyl cis-trans isomerase A (Ppia)	Down
							1. Ion channel 2. Mitochondrial apoptosis	Voltage-dependent anion-selective channel protein 2 (Vdac2)	Down
							Cytoplasmic tetramerisation domain of voltage-gated K+ channel	similar to Potassium channel tetramerisation domain containing protein 12	Up
				Spinal cord		19	Xenobiotic metabolism and cellular defense	Glutathione S-transferase P (Gstp1)	Down
							1. Glycolytic enzyme 2. Autoantigen in many diseases	α-enolase (Eno1)	Down
							Immunity	Proteasome subunit α type-1 (Psma1)	Down

DRG: Dorsal root ganglia; TNBS: Trinitrobenzene sulfonic acid; CD: Crohn's disease; UC: Ulcerative colitis; DSS: Dextran sulfate sodium; NF: Nuclear factor.