Proteome profiling of spinal cord and dorsal root ganglia in rats with trinitrobenzene sulfonic acid-induced colitis

Table 2 Proteins significantly regulated after trinitrobenzene sulfonic acid-induced colitis in spinal cord

Function	Cell component	Protein name	Abbreviation	AccessionNo.	TheoreticalPI/Mr (kDa)	Sequencecoverage(%)	MASSOTscore	Change(TNBS)
Proteins involved in inflammatory/immune response
1. Fatty acid catabolic process 2. Play a role in eicosanoid synthesis and inflammation	Cytoplasm	Isoform 1 of cytosolic acyl coenzyme A thioester hydrolase	Acot7	IPI00213571	7.16/37 936	14	167	↓
1. Glycolytic enzyme 2. Autoantigen	Cell membrane, cytoplasm	α-enolase	Eno1	IPI00464815	6.16/47 440	13	183	↓
Immunity	Cytoplasm, nucleus	Proteasome subunit α type-1	Psma1	IPI00191748	6.15/29 784	17	193	↓
Proteins involved in cell signaling
1. Growth protein 2. Modulate calcium influx 3. Regulate the release of ICGRP	Cytoplasm	Dihydropyrimidinase-related protein 2	Dpysl2	IPI00870112	5.95/62 638	16	270	↑
Calcium ion signaling	Cytoplasm, cytoskeleton	Troponin C-like protein		gi223036	4.12/16 696	10	91	↓
1. Cell division 2. Apoptosis 3. Regulate insulin sensitivity	Melanosome, cytoplasm	14-3-3 protein epsilon	Ywhae	IPI00325135	4.63/29 326	15	165	↑
Proteins involved in sulfate transport
Transferase detoxification	Mitochondrial matrix	Thiosulfate sulfurtransferase	Tst	IPI00366293	7.71/33 614	25	333	↓
Proteins involved in redox regulation
Xenobiotic metabolism and cellular defense	Nucleus	Glutathione S-transferase P	Gstp1	IPI00231229	6.89/23 652	20	263	↓
Proteins involved in chaperone function
Assist protein folding	Cytoplasm	T-complex protein 1 subunit γ	Cct3	IPI00372388	6.23/61 179	8	122	↑
Chaperonins/heat shock proteins	Mitochondria	Heat shock cognate 71 kDa protein	Hspa8	IPI00208205	5.37/71 055	11	172	↑
Chaperonins/heat shock proteins	Cytoplasm, nucleus	Stress-induced-phosphoprotein 1	Stip1	IPI00213013	6.4/63 158	7	143	↑
Proteins involved in cellular metabolism
Glycolytic enzyme	Axon, mitochondria	Fructose-bisphosphate aldolase C	Aldoc	IPI00231736	6.67/39 658	10	189	↓
Glycolytic enzyme	Cytoplasm	Similar to phosphoglycerate kinase 1	RGD1560402	IPI00372910	6.15/43 604	10	151	↑
1. Glycolytic enzyme 2. Transcription activation 3. Initiation of apoptosis	Cytoplasm	Similar to glyceraldehyde-3-phosphate dehydrogenase	RGD1565368	IPI00554039	8.44/36 045	18	454	↑
ATP transducing	Mitochondria	Creatine kinase, mitochondrial 1, ubiquitous	Ckmt1	IPI00555166	8.58/47 331	12	155	↑
1. Electron transport in respiratory chain 2. Oxidoreductase	Mitochondria	NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial	Ndufv2	IPI00367152	6.23/27 703	24	253	↑
Electron transport in respiratory chain	Membrane, mitochondria	NADH dehydrogenase (ubiquinone) 1 β subcomplex, 10	Ndufb10	IPI00202238	7.57/21 131	32	306	↓
1. Electron transport in respiratory chain 2. Mitochondrial dysfunction	Mitochondrial	Cytochrome b-c1 complex subunit 2, mitochondrial	Uqcrc2	IPI00188924	9.16/48 423	8	222	↓
Amino-acid (serine) biosynthesis		Phosphoserine aminotransferase	Psat1	IPI00331919	7.57/40 943	8	97	↓

↑: Elevated protein expression in TNBS group compared with saline group; ↓: Decreased protein expression in TNBS group in comparison with saline group; TNBS: Trinitrobenzene sulfonic acid; NADH: Nicotinamide adenine dinucleotide; Psma1: Proteasome subunit α type-1; Dpysl2: Dihydropyrimidinase-related protein 2; Ywhae: The 14-3-3 protein epsilon; Tst: Thiosulfate sulfurtransferase; Gstp1: Glutathione S-transferase P; Hspa8: Heat shock cognate 71 kDa protein; Stip1: Stress-induced phosphoprotein 1; Aldoc: Aldolase C; Ckmt1: Creatine kinase mitochondrial 1 ubiquitous; Ndufv2: NADH dehydrogenase (ubiquinone) flavoprotein 2; Ndufb10: NADH dehydrogenase (ubiquinone) 1 β subcomplex 10; Uqcrc2: Cytochrome b-c1 complex subunit 2; Psat1: Phosphoserine aminotransferase.