High dose glargine alters the expression profiles of microRNAs in pancreatic cancer cells

Figure 1 Effect of insulin glargine on miRNAs expression in Sw1990 cell line (A), insulin glargine up-regulated the expression of miR-95 and miR-134, miR-34c-3p showed no obvious changes in Sw1990 and Panc-1 cells (B). Ten miRNAs were significantly up-regulated and two miRNAs were down-regulated after 100 IU/L insulin glargine treatment for 48 h compared with non-treated cells. The cut off line for miRNA expression change was 2-fold. Data were presented as mean ± SD. Sw1990 and Panc-1 cells were incubated with increasing concentrations of insulin glargine (50-150 IU/L) for 48 h or treated with 100 IU/L insulin glargine for different periods (24-72 h). Then the expression of the three miRNAs were detected by stem-loop real-time reverse transcription-polymerase chain reaction array. miR-95 was significantly up-regulated, but no dose or time dependent changes were observed; miR-134 was up-regulated in a dose-dependent manner. miR-181a^*: The miRNA of lower abundance.