Key factors in developing the trinitrobenzene sulfonic acid-induced post-inflammatory irritable bowel syndrome model in rats

Figure 3 Effects of trinitrobenzene sulfonic acid administration position and ethanol percentage on severity of acute inflammation and inflammation resolution. Panel A depicts the appearance of colon tissue in rats treated with trinitrobenzene sulfonic acid (TNBS) at 4 cm or 8 cm from anus or in 25% ethanol. The colon tissues were collected at 5 d or 4 wk after TNBS administration, respectively; Statistical analysis of pathological score is shown in panel (B), and myeloperoxidase (MPO) activity in panel (C). Data are shown as mean ± SE, n = 4-5 per group. ^aP < 0.05 vs saline-treated rats, ^cP < 0.05 vs rats treated with TNBS at 8 cm from the anus in 50% ethanol (one-way analysis of variance, Student-Newman-Keuls).