Hexahydrocurcumin enhances inhibitory effect of 5-fluorouracil on HT-29 human colon cancer cells

Figure 5 Combination effects of hexahydrocurcumin and 5-fluorouracil on cyclooxygenase-2 protein expression in HT-29 colon cancer cells. A: Western blotting with polyclonal anti-cyclooxygenase (COX) 1 (70 kDa) and anti-COX2 (72 kDa), and anti-β actin (43 kDa) antibodies; B: Fold changes of proteins as represented below, each band was measured by Lab Image software. Combination treatment of 5-fluorouracil (5-FU) and hexahydrocurcumin (HHC) significantly decreased the expression of COX-2 protein with no effect on the expression of COX-1 protein. Values are expressed as percentage of control (mean ± SE), ^aP < 0.05 vs control; ^cP < 0.05 vs 5-FU or HHC monotherapy (n = 3).