Lactobacillus crispatus M206119 exacerbates murine DSS-colitis by interfering with inflammatory responses

Figure 4 Colonic cytokines changes in China Center for Type Culture Collection M206119 or vehicle-treated dextran sulfate sodium-colitis mice. Expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α from the colons of dextran sulfate sodium (DSS)-colitis mice treated with China Center for Type Culture Collection (CCTCC) M206119 or saline was measured by (A) RT-PCR and (B) western blotting at indicated time points; GAPDH was used as a loading control; C: IL-10 expression from colons of DSS-colitis mice treated with saline, 5-aminosalicylic acid (5-ASA), or CCTCC M206119 was measured by western blotting at indicated time points; β-actin was used as a loading control. The data represent at least three experiments. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.