Lactobacillus crispatus M206119 exacerbates murine DSS-colitis by interfering with inflammatory responses

Figure 3 Effects of China Center for Type Culture Collection M206119 strain on epithelial injury and translocation of nuclear factor-κB in vitro. Immunofluorescence staining for nuclear factor (NF)-κB p65 (red) and 4',6'-diamidino-2-phenylindole hydrochloride (DAPI) (blue) in HT-29 cells treated either by tumor necrosis factor (TNF)-α or China Center for Type Culture Collection (CCTCC) M206119 (scale bars, 10 mm). A: Increased positive NF-κB p65 staining after CCTCC M206119 treatment; B: Positive staining areas were quantified as pixels per high-power microscopic field (400 ×) using Photoshop. Error bars indicate mean ± SD (n = 3).