Lactobacillus crispatus M206119 exacerbates murine DSS-colitis by interfering with inflammatory responses

Figure 2 Alteration of fecal microflora in DSS-colitis mice after China Center for Type Culture Collection M206119 treatment. Bacterial counts of (A) aerobic (Escherichia coli, Staphylococcus, Bacteroides, total aerobic bacteria) and (B) anaerobic (Bifidobacterium, Lactobacillus, Enterococcus spp.) in colon luminal contents from healthy animals and dextran sulfate sodium (DSS)-colitis mice treated by saline, 5-aminosalicylic acid (5-ASA), or China Center for Type Culture Collection (CCTCC) M206119 strain at day 2 and day 7 after DSS-treatment, as determined by culture. The data sets were pooled from two independent experiments. Significance levels were determined by U test. Underlined P values shown in black were calculated from comparisons of healthy vs diseased animals; C: Zonula occludens (ZO)-1 and defensin 2 expression from colons of DSS-colitis mice treated with saline or CCTCC M206119 and healthy mice was measured by western blotting at indicated time points; GAPDH was used as a loading control. The data represent at least three experiments. ^aP < 0.05 vs healthy group. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.