Anti-tumor effect of 5-aza-2'-deoxycytidine by inhibiting telomerase activity in hepatocellular carcinoma cells

Figure 6 Demethylation of p16 promoter region and reactivation of p16 expression after 5-aza-2’-deoxycitidine treatment. Cells were incubated with 2 μmol/L DAC for 3-5 d and subjected to methylation-specific polymerase chain reaction (MSP) and real-time reverse transcription-polymerase chain reaction assay, respectively. M: Methylation; U: Unmethylation; Pos: Positive control. ^aP < 0.05 (by unpaired Student’s t test). DAC: 5-aza-2’-deoxycitidine.