Anti-tumor effect of 5-aza-2'-deoxycytidine by inhibiting telomerase activity in hepatocellular carcinoma cells

Figure 5 Effect of 5-aza-2’-deoxycitidine on expression of critical regulatory genes of telomerase reverse transcriptase transcription in hepatocellular carcinoma cell lines. A, B: Cells were incubated with 2 μmol/L DAC for 3-5 d, and subjected to real-time reverse transcription-polymerase chain reaction assay, ^aP < 0.05 (by unpaired Student’s t test); C: Western blotting analysis of c-myc and p16 in hepatocellular carcinoma cell lines by DAC. The same blot was reprobed for β-actin as a loading control. DAC: 5-aza-2’-deoxycitidine.