Matrix metalloproteinase-9 contributes to parenchymal hemorrhage and necrosis in the remnant liver after extended hepatectomy in mice

Figure 8 Effect of matrix metalloproteinase-9 monoclonal antibody on 80%-partial hepatectomy. A, B: Treatment with matrix metalloproteinase-9 monoclonal antibody (MMP-9 mAb) in mice with 80%-partial hepatectomy (PH) resulted in a significant reduction in the size (percent area) and number of hemorrhagic and necrotic foci 6 h after 80%-PH compared with treatment using control IgG (^aP < 0.05); C, D: Suppression of MMP-9 activity as determined by in situ fluorescence gelatin zymography compared with control IgG-treated mice; E, F: There was significantly less myeloperoxidase staining (arrows) in remnant liver sections in mice treated with MMP-9 mAb compared with that in control IgG-treated mice; G: A histogram shows the number of accumulated neutrophils in the remnant liver 6 h after 80%-PH. Significantly fewer neutrophils were observed in MMP-9 mAb-treated mice than in control IgG-treated animals (^aP < 0.05).