Matrix metalloproteinase-9 contributes to parenchymal hemorrhage and necrosis in the remnant liver after extended hepatectomy in mice

Figure 6 Expression and activity of matrix metalloproteinase-9. Expression and activity of matrix metalloproteinase (MMP)-9 were determined using (A) western blotting analysis for MMP-9 with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a control, (B) polyacrylamide gel electrophoresis gelatin zymography with human MMP-9 and -2 standards (S), and in situ fluorescence gelatin zymography in the remnant livers of wild-type (WT), MMP-9(-/-), and tissue inhibitors of metalloproteinases (TIMP)-1(-/-) mice at 6 h after 80%-partial hepatectomy. We observed MMP-9 protein expression and activity in WT mice (C), but they were absent in MMP-9(-/-) mice (D). Enhancement of MMP-9 protein expression and activity in TIMP-1(-/-) mice was observed (E).