Matrix metalloproteinase-9 contributes to parenchymal hemorrhage and necrosis in the remnant liver after extended hepatectomy in mice

Figure 4 Co-localization analysis by dual immunofluorescence in the remnant liver after 80%-partial hepatectomy. Co-localization analysis by dual immunofluorescence in the remnant liver for (A) matrix metalloproteinase (MMP)-9 labeled in red (Alexa Fluor 568), (B) CD68 labeled in green (Alexa Fluor 488), and (C) both MMP-9 and CD68; for (D) MMP-9 labeled in red (Alexa Fluor 568), (E) desmin labeled in green (Alexa Fluor 488), and (F) both MMP-9 and desmin; and for (G) MMP-9 labeled in red (Alexa Fluor 568), (H) CD11b labeled in green (Alexa Fluor 488), and (I) both MMP-9 and CD11b. These co-localization results demonstrate that MMP-9 protein expression is mainly localized in CD11b-positive cells, and to a lesser extent in desmin-positive cells.