CD74 and macrophage migration inhibitory factor as therapeutic targets in gastric cancer

Figure 2 Lipopolysaccharide stimulation induced migration inhibitory factor and surface CD74 expression in gastric cancer cell line MKN-45. A: MKN-45 cell line was stimulated with lipopolysaccharide (LPS) at the indicated concentration respectively for 24 h, the supernatants were collected and migration inhibitory factor (MIF) concentration was measured by enzyme-linked immunosorbent assay; B and C: MKN-45 cell line was stimulated with or without LPS (1 μg/mL) for 24 h, toll-like receptor 4 (TLR4) (B) and CD74 surface expression was detected by flow cytometry (C, left panel), the mean values of CD74-positive cells were compared between the medium and condition group (right panel). ^bP < 0.01 vs medium group.