Affinity peptide developed by phage display selection for targeting gastric cancer

Figure 4 Immunofluorescence analysis of fluorescein isothiocyanate-conjugated AADNAKTKSFPV binding to human gastric cancer tissues. Frozen sections for biopanning were incubated with fluorescein isothiocyanate-conjugated AADNAKTKSFPV (AAD); scrambled peptide PAKFKAANSDVT was used as the control. Immunofluorescence stain with FITC-conjugated AAD showed selective signals (green) located in tumor membrane, cytoplasm (C), but no binding to normal gastric mucosae (B). As a control, the scramble peptide displayed no signals in tumor tissues (A).