Transactivation of the TIEG1 confers growth inhibition of transforming growth factor-β-susceptible hepatocellular carcinoma cells

Figure 4 The overexpression of transforming growth factor-β-inducible early gene 1 by lentiviral-mediated transfection-induced growth inhibition and apoptosis in transforming growth factor-β1-resistant hepatoma Bel7404 and HepG2 cells. A and B: Increases in mRNA and protein levels of transforming growth factor (TGF)-β-inducible early gene 1 (TIEG1) in Bel7404 and HepG2 cells transfected by lenti-TIEG1, respectively, revealed by reverse transcriptase-polymerase chain reaction and Western blotting analysis; C: The overexpression of TIEG1 by lentiviral-mediated transfection-inhibited cell growth in Bel7404 and HepG2 cells. Cell survival was determined by methylthiazoletetrazolium assays; D: Induction of apoptosis in Bel7404 and HepG2 cells after lenti-TIEG1 transfection for 72 h as shown by 4’,6-Diamidino-2-phenylindole staining. Scale bar: 50 μm, 400 ×.