Study of Helicobacter pylori genotype status in saliva, dental plaques, stool and gastric biopsy samples

Table 1 Primers used for polymerase chain reaction analysis of voculating cytotoxin gene A and cytotoxin associated gene A

Region	Primer	Sequence (5'-3')	Size and location of PCR product
s1a	vacA s1a-F	CTC TCG CTT TAG TAG GAG C	213 bp
	VA1-R	CTG CTT GAA TGC GCC AAA C	(843-1055)
s1b	SS3-F	AGC GCC ATA CCG CAA GAG	187 bp
	VA1-R	CTG CTT GAA TGC GCC AAA C	(869-1055)
s1c	vacA s1c-F	CTC TCG CTT TAG TGG GGY T	213 bp
	VA1-R	CTG CTT GAA TGC GCC AAA C	(843-1055)
s2	SS2-F	GCT AAC ACG CCA AAT GAT CC	199 bp
	VA1-R	CTG CTT GAA TGC GCC AAA C	(433-631)
m1a	VA3-F	GGT CAA AAT GCG GTC ATG G	290 bp
	VA3-R	CCA TTG GTA CCT GTA GAA AC	(2741-3030)
m1b	VAm-F3	GGC CCC AAT GCA GTC ATG GA	291 bp
	VAm-R3	GCT GTT AGT GCC TAA AGA AGC AT	(2741-3031)
m2	VA4-F	GGA GCC CCA GGA AAC ATT G	352 bp
	VA4-R	CAT AAC TAG CGC CTT GCA	(976-1327)
cagA	cagA-U	GGA ATA CCA AAA ACG CAA AAA CCA	300 bp
	cagA-L	CCC CAC AAT ACA CCA GCA AAA CT
ureC (glmM)	GlmM1-R	GCTTACTTTCTAACACTAACGCGC	296 bp
	GlmM1-F	GGATAAGCTTTTAGGGGTGTTAGGGG

PCR: Polymerase chain reaction; cagA: Cytotoxin associated gene A; vacA: Voculating cytotoxin gene A.