Aberrant methylation of SPARC in human hepatocellular carcinoma and its clinical implication

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May 7, 2012 (publication date) through Jul 7, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 7, 2012; 18(17): 2043-2052
Published online May 7, 2012. doi: 10.3748/wjg.v18.i17.2043

Table 1 Primer sequences for polymerase chain reaction

Gene	Primer sequences (forward/reverse 5’-3’)	Accession No.	Location to transcription start	Product size (bp)	Annealing temperature (°C)
SPARC methylation	GAGAGCGCGTTTTGTTTGTC	NM_003118.2	+52 to +71	112	54
	AACGACGTAAACGAAAATATCG		+142 to +163
SPARC unmethylation	TTTTTTAGATTGTTTGGAGAGTG	NM_003118.2	+36 to +58	132	59
	AACTAACAACATAAACAAAAATATC		+143 to +167
SPARC BS	GATAGAGATAGTTTTGGTTATGGGA	NM_003118.2	-119 to -95	401	55
	CCACCTTCTAAAAAACA ACAAAC		+260 to +282
SPARC mRNA	CGCATGCGGGACTGGCTCAA	NM_003118.2	+601 to +620	148	60
	GCTCCACGGGG TGGTC TCCT		+729 to +748
GAPDH mRNA	GGGCATCCTGGGCTACACTGA	NM_002046.3	+915 to +935	143	58
	CAAATTCGTTGTCATACCAGGAAATG		+1032 to +1057

SPARC: Secreted protein acidic and rich in cysteine; BS: Bisulfite sequencing; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.

Citation: Zhang Y, Yang B, Du Z, Bai T, Gao YT, Wang YJ, Lou C, Wang FM, Bai Y. Aberrant methylation of SPARC in human hepatocellular carcinoma and its clinical implication. World J Gastroenterol 2012; 18(17): 2043-2052
URL: https://www.wjgnet.com/1007-9327/full/v18/i17/2043.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i17.2043