Aberrant methylation of SPARC in human hepatocellular carcinoma and its clinical implication

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 18, Issue 17

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

All Articles published online

Item

Count

PDF

441

HTML

4098

Figures (1-6)

252

Tables (1-7)

235

Sum=5026

May 7, 2012 (publication date) through Dec 4, 2024

Times Cited of This Article

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Original Article

World J Gastroenterol. May 7, 2012; 18(17): 2043-2052
Published online May 7, 2012. doi: 10.3748/wjg.v18.i17.2043

Table 1 Primer sequences for polymerase chain reaction

Gene	Primer sequences (forward/reverse 5’-3’)	Accession No.	Location to transcription start	Product size (bp)	Annealing temperature (°C)
SPARC methylation	GAGAGCGCGTTTTGTTTGTC	NM_003118.2	+52 to +71	112	54
	AACGACGTAAACGAAAATATCG		+142 to +163
SPARC unmethylation	TTTTTTAGATTGTTTGGAGAGTG	NM_003118.2	+36 to +58	132	59
	AACTAACAACATAAACAAAAATATC		+143 to +167
SPARC BS	GATAGAGATAGTTTTGGTTATGGGA	NM_003118.2	-119 to -95	401	55
	CCACCTTCTAAAAAACA ACAAAC		+260 to +282
SPARC mRNA	CGCATGCGGGACTGGCTCAA	NM_003118.2	+601 to +620	148	60
	GCTCCACGGGG TGGTC TCCT		+729 to +748
GAPDH mRNA	GGGCATCCTGGGCTACACTGA	NM_002046.3	+915 to +935	143	58
	CAAATTCGTTGTCATACCAGGAAATG		+1032 to +1057

SPARC: Secreted protein acidic and rich in cysteine; BS: Bisulfite sequencing; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.

Citation: Zhang Y, Yang B, Du Z, Bai T, Gao YT, Wang YJ, Lou C, Wang FM, Bai Y. Aberrant methylation of SPARC in human hepatocellular carcinoma and its clinical implication. World J Gastroenterol 2012; 18(17): 2043-2052
URL: https://www.wjgnet.com/1007-9327/full/v18/i17/2043.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i17.2043