Probiotic metabolites from Bacillus coagulans GanedenBC30TM support maturation of antigen-presenting cells in vitro

Figure 3 Expression of the co-stimulatory molecules CD80 and CD86 on CD14^dim mononuclear phagocytes from 3-d peripheral blood mononuclear cell cultures. A: Comparison between the effects of serial dilutions of Ganeden Bacillus coagulans 30 (GBC30) crude metabolites (MET) or cell wall enriched (CW) fractions on CD80 expression on CD14^dim cells showed that both MET and CW led to increased expression; B: The increase in CD80 expression following treatment of cells with MET was due to high molecular weight compounds; C: Comparison of CD86 expression on CD14^dim cells exposed to crude fractions of MET or CW resulted in increased CD86 expression; D: Size-selected fractions of MET did not have uniform effects on CD86 expression on CD14^dim cells. Crude MET increased expression while 3-30 kDa and < 3 kDa fractions decreased expression. Bar graphs show data from 1:200 dilutions of each MET fraction and lipopolysaccharide (1 μg/mL). ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001. For each data point, the mean + SD are shown for each duplicate data set. Graphs show data representative of 1 out of 3 experiments. MFI: Mean fluorescence intensity; UT: Untreated; LPS: Lipopolysaccharide.