Probiotic metabolites from Bacillus coagulans GanedenBC30TM support maturation of antigen-presenting cells in vitro

Figure 2 Expression of the co-stimulatory molecules CD80 and CD86 on CD14^bright mononuclear phagocytes from 3-d peripheral blood mononuclear cell cultures. A: Comparison between the effects of serial dilutions of Ganeden Bacillus coagulans 30 (GBC30) crude metabolites (MET) or cell wall enriched (CW) fractions on CD80 expression on CD14^bright cells showed dose-dependent decreases in CD80 expression. Both MET and CW reduced CD80 expression to levels similar to those seen with Lipopolysaccharide (LPS) treatment; B: A comparison of the effects of size-fractionated MET on CD80 expression on CD14^bright cells shows that all MET fractions reduce expression; C: Comparison between the effects of serial dilutions of crude MET or CW on CD86 expression on CD14^bright cells showed dose-dependent increases in CD86 expression when cells were exposed to the three most concentrated dilutions of MET. Treatment of cells with CW resulted in a uniform modest decrease in CD86 expression; D: The effect on increased CD86 expression is present only in the crude preparation of MET. Bar graphs show data from 1:200 dilutions of each MET fraction and lipopolysaccharide (1 μg/mL). ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001. For each data point, the mean + SD are shown for each duplicate data set. Graphs show data representative of 1 out of 3 experiments. MFI: Mean fluorescence intensity; UT: Untreated.