Glycer-AGEs-RAGE signaling enhances the angiogenic potential of hepatocellular carcinoma by upregulating VEGF expression

Figure 5 Effect of glyceraldehyde-derived advanced glycation end-products-treated CM on human umbilical vein endothelial cells proliferation. Cell viability was determined with the WST-8 assay. Human umbilical vein endothelial cells were incubated with control unglycated bovine serum albumin (BSA), glyceraldehyde-derived advanced glycation end-products (Glycer-AGEs) (100 μg/mL), CM-BSA, or CM-Glycer-AGEs for 72 h. The open and filled bars represent results for cells treated with control unglycated BSA and Glycer-AGEs, respectively, and the light grey and the black grey bars represent results for cells treated with CM-BSA and CM-Glycer-AGEs, respectively. Data are shown as the mean ± SD (n = 6), ^bP < 0.01 vs CM-BSA.