Pro-apoptotic effects of tectorigenin on human hepatocellular carcinoma HepG2 cells

Figure 8 Effects of tectorigenin on integrity of mitochondrial membrane in HepG2 cells. Cells were treated with tectorigenin for different time points, then incubated with Rhodamine 123 and analyzed by flow cytometry. A: HepG2 cells were treated with 0 (a), 5 (b), 10 (c) and 20 (d) mg/L tectorigenin for 24 h. Mitochondrial membrane potential was measured by Rh123, a fluorescent probe, on FACSCalibur at FL2 channel with an excitation wavelength of 488 nm. Each test was performed 3 times and images presented were typical of 3 independent experiments; B: The data were presented as mean ± SD (n = 3). Difference was considered statistically significant when ^aP < 0.05 and ^bP < 0.01 vs control.