Pro-apoptotic effects of tectorigenin on human hepatocellular carcinoma HepG2 cells

Figure 5 Flow cytometric analysis of apoptosis in HepG2 cells treated with tectorigenin. HepG2 cells were incubated for 24 and 48 h with tectorigenin at 0, 5, 10, 20 mg/L, respectively. And then the cells were stained with EGFP-conjugated Annexin V and propidium iodide (PI). The EGFP and PI fluorescence was measured using flow cytometer with FL1 and FL3 filters, respectively. A: Representative dot plots of Annexin V/PI staining. a: Control, 24 h; b: 5 mg/L, 24 h; c: 10 mg/L, 24 h; d: 20 mg/L, 24 h; e: Control, 48h; f: 5 mg/L, 48 h; g: 10 mg/L, 48 h; h: 20 mg/L, 48 h. The lower left quadrant contains the vital (double negative) population. The lower right quadrant contains the early apoptotic (Annexin V⁺/PI^-) population and upper right quadrant contains the late apoptotic/necrotic (Annexin V⁺/PI⁺) population; B: Data pooled from three independent experiments show the percentage of apoptotic cells. Difference was considered statistically significant when ^aP < 0.05 and ^bP < 0.01 vs control.