Macrophage secretory products induce an inflammatory phenotype in hepatocytes

Figure 5 Matrix metalloproteinase-9 mRNA expression is significantly increased in macrophages compared with THP-1 monocytes. A: Results are expressed as fold of monocytes (mean ± SEM, n = 6) (P < 0.05 vs monocytes); B: Western blotting analysis (top) and zymography (bottom) of matrix metalloproteinase (MMP)-9 in MonoCM and macrophage-conditioned media (MφCM) from three independent experiments; C: Generation of MφCM in the presence of MMP-9 inhibitor I (100 μmol) prevented the MφCM-induced morphological change in HepG2 cells; D: Zymography gel confirming MMP-9 inhibitor I reduces MMP-9 activity in MφCM at 100 μmol; E-G: MMP-9 Inhibitor I significantly attenuated downregulation of E-cadherin (E) and upregulation of transforming growth factor-β1 (TGF-β1) (F) but not lipocalin-2 (LCN2) (G) mRNA expression in response to MφCM. Results are expressed as fold of untreated cells (mean ± SEM, n = 5), P < 0.05 vs untreated.