Wnt5a participates in hepatic stellate cell activation observed by gene expression profile and functional assays

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Apr 21, 2012 (publication date) through Jul 29, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 21, 2012; 18(15): 1745-1752
Published online Apr 21, 2012. doi: 10.3748/wjg.v18.i15.1745

Table 1 Primer sequences of genes validating the microarray analysis by quantitative real-time polymerase chain reaction

Gene name	Sense primer	Antisense primer
Wnt5a	AGGGCTCCTACGAGAGTGCT	GACACCCCATGGCACTTG
Frizzled2	TCTCTGAGGACGGTTATCGCA	CAGAATCACCCACCAGATGGA
Calcium calmodulin mediated kinase IIdelta	TCGGCTCACACAGTACATGGA	CCCCGAACGATGAAAGTGAA
α-1 type I collagen	ACAGACTGGCAACCTCAAGAAG	AAGCGTGCTGTAGGTGAATCG
Connective tissue growth factor	GTTGGCGAACAAATGGCCTT	TGCCTCCCA AACCAGTCATAG
Glyceraldehydes-3-phosphate dehydrogenase	TCCTGCACCACCAACTGCTTAG	AGTGGCAGTGATGGCATGGACT

Citation: Xiong WJ, Hu LJ, Jian YC, Wang LJ, Jiang M, Li W, He Y. Wnt5a participates in hepatic stellate cell activation observed by gene expression profile and functional assays. World J Gastroenterol 2012; 18(15): 1745-1752
URL: https://www.wjgnet.com/1007-9327/full/v18/i15/1745.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i15.1745