Macrophage secretory products induce an inflammatory phenotype in hepatocytes

Table 2 Primer and probe sequences used in real-time polymerase chain reaction assays

E-cadherin	for	5’-ATTGCAAATTCCTGCCATTC-3’
	rev	5’-GCTGGCTCAAGTCAAAGTCC-3’
Vimentin	for	5’-GTTTCCAAGCCTGACCTCAC-3’
	rev	5’-GCTTCAACGGCAAAGTTCTC-3
TGF-β1	for	5’-AAGTGGACATCAACGGGTTC-3’
	rev	5’-TGCGGAAGTCAATGTACAGC-3
MMP-9	for	5’-TTCGACGTGAAGGCGCAGATGG-3’
	rev	5’-AACTCACGCGCCAGTAGAAGCG-3’
CD163	for	5’-CCAACAAGATGCTGGAGTGAC-3’
	rev	5’-TGACAGCACTTCCACATTCAAG-3’
Lipocalin-2	for	5’-TCACCCTCTACGGGAGAACCAAGG-3’
	rev	5’-TGTGCACTCAGCCGTCGATACAC-3’
GAPDH	for	5’-TGCACCACCAACTGCTTAGC-3’
	rev	5’-GGCATGGACTGTGGTCATGAG-3’
	probe	5’-CCTGGCCAAGGTCATCCATGACAACTT-3’
HuPO	for	5’-GCTTCCTGGAGGGTGTCC3-3’
	rev	5’-GGACTCGTTTGTACCCGTTG-3’
	probe	5’-TGCCAGTGTCTGTCTGCAGATTGG-3’
18s	for	5’-GCCCGAAGCGTTTACTTTGA-3’
	rev	5’-TCCATTATTCCTAGCTGCGGTATC-3’
	probe	5’-AAAGCAGGCCCGAGCCGCC-3’

E-cadherin: Epithelial cadherin; TGF-β1: Transforming growth factor-β1; MMP-9: Matrix metalloproteinase-9; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; 18s: 18S ribosomal RNA; HuPO: Human acidic ribosomal protein; for: Forward; rev: Reverse.