Overexpression of Dickkopf-3 induces apoptosis through mitochondrial pathway in human colon cancer

Figure 2 pEGFP-N1-Dkk-3-GFP plasmid induces overexpression of Dickkopf-3 in human colon cancer LoVo cells. A: Semi-quantitative reverse transcription polymerase chain reaction of RNA extracted from pEGFP-N1-Dkk-3-GFP plasmid transfected LoVo cells and the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was amplified as a control; B: Immunoblotting of total protein lysates extracted from pEGFP-N1-Dkk-3-GFP plasmid transfected LoVo cells, and actin was included as a loading control; C: Green fluorescent protein (GFP) was also detected under a fluorescence microscope in pEGFP-N1-Dkk-3-GFP plasmid transfected LoVo cells (× 400). ^aP < 0.05 vs LoVo or pEGFP-N1 using Student’s t test.