Increased presence of effector lymphocytes during Helicobacter hepaticus-induced colitis

Figure 4 Changes in effector T lymphocyte populations in the mesenteric lymph nodes of drosophila mothers against decapentaplegic 3^+/- and drosophila mothers against decapentaplegic^-/- mice following infection with Helicobacter hepaticus. Flow cytometric analysis of lymphocyte populations at days 0, 3, 7 and 28 post-infection. Gates were drawn on viable cells using forward scatter vs side scatter parameters. Histograms represent CD62L expression at 0 d (solid grey), 3 d (dotted black), 7 d (dashed black) and 28 d (solid black) post-infection. Y-axis represents relative cell frequency. X-axis is CD62L expression. Brackets indicate CD62L^lo gate used to determine cell percentages. A: Total CD8⁺ CD62L^lo lymphocytes gated on CD3⁺/CD8⁺ lymphocytes; B: CD62L expression in drosophila mothers against decapentaplegic (SMAD)3^+/- CD8⁺ lymphocytes; C: CD62L expression in SMAD3^-/- CD8⁺ lymphocytes; D: Total CD4⁺ CD62L^lo lymphocytes gated on CD3⁺/CD4⁺ lymphocytes; E: CD62L expression in SMAD3^+/- CD4⁺ lymphocytes; F: CD62L expression in SMAD3^-/- CD4⁺ lymphocytes; G: Total CD25^+/FOXP3⁺ CD62L^lo Treg cells gated on CD3⁺/CD4⁺ lymphocytes (n = 4-6 animals per time point); H: CD62L expression in SMAD3^+/- CD25⁺/FOXP3⁺ lymphocytes; I: CD62L expression in SMAD3^-/- CD25⁺/FOXP3⁺ lymphocytes. ^aP < 0.05 vs baseline values; ^cP < 0.01 denotes significant interaction between genotypes (SMAD3^-/-vs SMAD3^+/–).