Notch3 regulates the activation of hepatic stellate cells

Figure 5 Knockdown of Notch3 decreased the secretion of myofibroblastic marker in hepatic stellate cell-T6. A: Effects of Notch3 knockdown in HSC-T6 using Notch3 siRNA (N3 si), and control siRNA (Cont si) were investigated. Expression of Notch3, α-smooth muscle actin (SMA) and collagen I was detected by Western blotting. Graphic representation of relative level of Notch3, α-SMA and collagen I normalized to β-actin; B: The expression of α-SMA and collagen I was also detected by immunofluorescence staining (x 400). ^aP < 0.05 vs control siRNA group, ^cP < 0.05 vs transforming growth factor (TGF)-β1+control siRNA group. 1: Cont si; 2: N3 si; 3: TGF-β1+N3 si; 4: TGF-β1+Cont si.