Polymorphisms of the TLR2 and TLR4 genes are associated with risk of gastric cancer in a Brazilian population

Table 1 Primer sequences, restriction enzymes and fragment sizes for toll-like receptor 2 and toll-like receptor 4 gene polymorphisms and interleukin-1β gene

Genes	Primers	Enzyme T°/time	Fragment (bp)	Ref.
TLR2	F: 5’-CACGGAGGCAGCGAGAAA-3’	-	286	[24]
del -196 to -174	R: 5’-CTGGGCCGTGCAAAGAAG-3’		ins/ins: 286
			ins/del: 286, 264
			del/del: 264
TLR4+896A/G	F: 5’-AGCATACTTAGACTACCACCTCGATG 3’	BstXI	131	[34]
rs4986790	R: 5’-GTTGCCATCCGAAATTATAAGAAAAG 3’	37 °C, 1 h	A/A: 131
			A/G: 131, 108
			G/G: 108
TLR4+1196C/T	F: 5’-GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA-3’	HinfI	407	[33]
rs4986791	R: 5’-ACCTGAAGACTGGAGAGTGAGTTAAATGCT-3’	37 °C, 1 h	C/C: 407
			C/T: 407, 378
			T/T: 378
IL1-β	F: CATGTGACCTGCTCGTCAGT	HinfI	370	[47]
	R: CCCTAGGGATTGAGTCCACA	37 °C, 1 h	195, 175
TLR2	F: 5’-CACGGAGGCAGCGAGAAA-3’	BstXI	286	[24]
	R: 5’-CTGGGCCGTGCAAAGAAG-3’	37 °C, 1 h	188, 98

TLR: Toll-like receptor; IL: Interleukin; T^o: Temperature.