Hepatoma cell line HepG2.2.15 demonstrates distinct biological features compared with parental HepG2

Figure 4 Tumor formation of HepG2 and HepG2. 2.15 cells in vivo. A: The volume of subcutaneous tumors were measured and recorded every 2 d. The difference of tumor growth rate between HepG2 and HepG2.2.15 groups was significant (P < 0.01, Mann-Whithey U test); B: Tumor development in four groups in vivo; C: Expansive growth of tumor. The boundary of tumor and normal tissue is clear [hematoxylin and eosin (HE) stain, × 120]. T: Tumor; H: Liver tissue; D: Fatty changes in liver tissue of HepG2.2.15 groups (HE stain, × 120); E: Lung metastasis in HepG2 injected group (HE stain, × 460) and low power field.