Hepatoma cell line HepG2.2.15 demonstrates distinct biological features compared with parental HepG2

Figure 3 Invasion assays of HepG2 and HepG2. 2.15 cells. The trans-well membranes were collected at different time points and the cells that went through the pores were stained with hematoxylin and eosin. The cell numbers were counted. A: HepG2 cells collected and stained at 2 h, 4 h, 6 h and 12 h after incubation; B: HepG2.2.15 cells collected and stained at 24 h, 36 h, 48 h and 60 h after incubation; C: Western blotting analysis of F-actin and Ezrin.