Hepatoma cell line HepG2.2.15 demonstrates distinct biological features compared with parental HepG2

Figure 2 Cell proliferation and apoptosis flow cytometry. A: The levels of hepatitis B surface antigen (HBsAg) and hepatitis B envelope antigen (HBeAg) in HepG2.2.15 cell supernatant. The supernatant was collected every 24 h and tested by enzyme-linked immunosorbent assay; B: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay of cell proliferation. The absorbencies of test wells were read every 24 h and the data represent the mean ± SD (^bP < 0.001); C: Flow cytometry of cell cycle; D: Apoptosis percentages in B1, B2 and B4 areas. All experiments were repeated three times with similar results.